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Find the best regional variations for the movies you enjoy on IMDbPro Free.The present invention relates to a stranding machine and method, and more particularly to a machine and method for stranding wire stock with the ends of successive lengths of the wire stock remaining interconnected.
Stranders for removing the exposed end portion of wire stock have been proposed heretofore. Such stranders are illustrated in the U.S. Pat. Nos. 3,106,226; 3,335,575; 3,476,731; 3,618,135 and 3,675,805.
The machines illustrated in the referenced patents involve considerable complexity in the equipment required to engage the wire stock with various drive means. The relatively complex machines illustrated in the referenced patents require a significant amount of maintenance to insure reliability and they tend to be relatively costly items to operate. Furthermore, the machines illustrated in the referenced patents are not suitable for applying a protective coating on the ends of the wire stock.Human leukocyte antigen-B27 genotyping: a rapid and automated system using microcolumns.
Human leukocyte antigen (HLA)-B27 genotyping is increasingly performed in many laboratories. Automated HLA-B27 genotyping was developed to replace HLA-B27 serology which is cumbersome, time consuming, and expensive. We explored whether HLA-B27 genotyping could be automated by using the Kofler microcolumn technique. Human leukocyte antigen-B27 genotypes were determined from genomic DNA by using allele-specific polymerase chain reaction (PCR) amplification followed by hybridization to allele-specific immobilized oligonucleotide probes bound to nitrocellulose microcolumns. Sixty-seven samples were tested in parallel by the microcolumn method and the conventional agarose-gel electrophoresis (A-E) method. Genotypes were obtained by direct visual comparison of the microcolumn chromatographs and electrophoretograms. Polymorphism was verified by genotyping of family members. Success rates were 90% and 96% for HLA-B27(1) and B27(2) samples, respectively. The microcolumn method was 5 to 10 times less time consuming than A-E. This automated system should increase the capacity of HLA-B27 genotyping while reducing labor and equipment costs.The