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In this study, we investigated changes in the olfactory pathway of adult rats after the injection of 50 microg, 100 microg, or 200 microg of kainic acid or 2 mg of colchicine into the right olfactory bulb. After 1, 3, or 7 days of injections, the rats were killed, and olfactory pathways were examined for the following parameters: protein content in the olfactory mucosa, the thickness of the olfactory mucosa, total length of the olfactory nerve, cross-sectional area of the nerve, the number of nerve fibres, and total length of the nasal cavity. Immunohistochemical analysis was performed for glial fibrillary acidic protein (GFAP), nerve growth factor (NGF), and glial acidic fibrillary protein (GFAF). After the injection of kainic acid, olfactory nerve thickness decreased at the lower dose, but increased at the higher doses. After the injection of colchicine, olfactory nerve thickness decreased at all doses. Concomitant with these changes, the NGF-positive area decreased and the GFAP-positive area and the GFAF-positive area increased. We also observed downregulation in the olfactory nerve and a decrease in the number of nerve fibres, but the mean lengths of these nerves did not change. These results suggest

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